Effects of laser Therapy on Morselized Bone Allograft Incorporation Laser Therapy and Bone Grafts Incorporation

Objective: To assess the effect of low-level laser therapy (LLLT) on morselized bone allograft incorporation. Material and Methods: Four rabbits were used as bone tissue donors. Morselized bone allografts and autografts were placed into the calvaria of 20 rabbits divided across four groups: two LLLT groups (allograft, autograft and blood clot) and two non-irradiated control groups (allograft, autograft and blood clot). Animals were euthanized at 35 days (n = 10) or 70 days (n = 10). LLLT consisted of spot laser irradiation (GaAlAs diode laser, λ = 830 nm, energy density 4 J/cm2) over four points on the skull, for a total of 16 J/cm2 per session and a total treatment dose of 128 J/cm2. Results: At 70 days, animals in the allograft + LLLT group had statistically significant differences in bone remodeling (p = 0.008) and vascularization (p = 0.032) as compared with the allograft control group. There were no statistically significant differences in bone incorporation in the allograft + LLLT and auto graft control groups. Conclusion: Light microscopy and scanning electron microscopy analysis showed a qualitatively and quantitatively positive effect of LLLT on the speed of osteogenesis after morselized bone allograft placement.